Professor Neal Stewart Publications

  

Back to Racheff Chair of Excellence

2022 Publications


Piya, S., Hawk, T., Patel, B., Baldwin, L., Rice, J.H., Stewart, C.N. & et al (2022) Kinase-dead mutation: A novel strategy for improving soybean resistance to soybean cyst nematode Heterodera glycinesMolecular Plant Pathology, 23, 417– 430. https://doi.org/10.1111/mpp.13168

Occhialini, A., Pfotenhauer, A. C., Li, L., Harbison, S. A., Lail, A. J., Burris, J. N., Piasecki, C., Piatek, A. A., Daniell, H., Stewart, C. N. and Lenaghan, S. C. (2022) Mini-synplastomes for plastid genetic engineering. Plant Biotechnol. J.https://doi.org/10.1111/pbi.13717

Occhialini A. et al. (2022) High-Throughput Transfection and Analysis of Soybean (Glycine max) Protoplasts. In: Wang K., Zhang F. (eds) Protoplast Technology. Methods in Molecular Biology, vol 2464. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2164-6_17

2021 Publications


Xu, Y.; Shrestha, V.; Piasecki, C.; Wolfe, B.; Hamilton, L.; Millwood, R.J.; Mazarei, M.; Stewart, C.N. Sustainability Trait Modeling of Field-Grown Switchgrass (Panicum virgatum) Using UAV-Based Imagery. Plants 2021, 10, 2726. https://doi.org/10.3390/plants10122726

Piya, S., Hawk, T., Patel, B., Baldwin, L., Rice, J.H., Stewart, C.N. & et al (2021) Kinase-dead mutation: A novel strategy for improving soybean resistance to soybean cyst nematode Heterodera glycinesMolecular Plant Pathology, 00, 1– 14. https://doi.org/10.1111/mpp.13168

Wei, W., & Stewart, C. N. (2022). Gene flow: Monitoring, modeling and mitigation. CABI Publication.

Sultana, M.S., Millwood, R.J., Mazarei, M. et al. Proteinase inhibitors in legume herbivore defense: from natural to genetically engineered protectants. Plant Cell Rep (2021). https://doi.org/10.1007/s00299-021-02800-7

Balasubramanian, V. K., Purvine, S. O., Liang, Y., Kelly, R. T., Pasa-Tolic, L., Chrisler, W. B., Blumwald, E., Stewart, C. N., Zhu, Y., & Ahkami, A. H. (2021). Cell-type-specific proteomics analysis of a small number of plant cells by integrating laser capture microdissection with a nanodroplet sample processing platform. Current Protocols, 1, e153. doi: 10.1002/cpz1.153

Yang, Y.; Gardner, C.; Gupta, P.; Peng, Y.; Piasecki, C.; Millwood, R.J.; Ahn, T.-H.; Stewart, C.N., Jr. Novel Candidate Genes Differentially Expressed in Glyphosate-Treated Horseweed (Conyza canadensis). Genes 2021, 12, 1616. https://doi.org/10.3390/genes12101616

Occhialini, A., Pfotenhauer, A.C., Li, L., Harbison, S.A., Lail, A.J., Burris, J.N., Piasecki, C., Piatek, A.A., Daniell, H., Stewart, C.N., Jr and Lenaghan, S.C. (2021), Mini-synplastomes for plastid genetic engineering. Plant Biotechnology Journal. Accepted Author Manuscript. https://doi.org/10.1111/pbi.13717

Balasubramanian, V. K., Purvine, S. O., Liang, Y., Kelly, R. T., Pasa-Tolic, L., Chrisler, W. B., Blumwald, E., Stewart, C. N., Zhu, Y., & Ahkami, A. H. (2021). Cell-type-specific proteomics analysis of a small number of plant cells by integrating laser capture microdissection with a nanodroplet sample processing platform. Current Protocols, 1, e153. doi: 10.1002/cpz1.153

Guan, Z.-J., W. Wei, C.N. Stewart, Jr. Z-X. Tang. 2021. Effects of transgenic oilseed rape harboring the Cry1Ac gene on microbial communities in the rhizosphere soil. European Journal of Soil Biology 103: 103277.   https://doi.org/10.1016/j.ejsobi.2021.103277

Rigoulot, S. B., Schimel, T. M., Lee, J. H., Sears, R. G., Brabazon, H., Layton, J. S., Li, L., Meier, K. A., Poindexter, M. R., Schmid, M. J., Seaberry, E. M., Brabazon, J. W., Madajian, J. A., Finander, M. J., DiBenedetto, J., Occhialini, A., Lenaghan, S. C.and Stewart, C. N. (2021) Imaging of multiple fluorescent proteins in canopies enables synthetic biology in plants. Plant Biotechnol Jhttps://doi.org/10.1111/pbi.13510

Yang, Y., Lee, J. H., Poindexter, M. R., Shao, Y., Liu, W., Lenaghan, S. C., Ahkami, A. H., Blumwald, E.and Stewart, C. N. (2021) Rational design and testing of abiotic stress-inducible synthetic promoters from poplar cis-regulatory elements. Plant Biotechnol. J.https://doi.org/10.1111/pbi.13550

2020 Publications


Alexander, L, C. Hatcher, M. Mazarei, E. Haynes. H. Baxter, K. Kim, C. Hamilton, R. Sykes, G. Turner, M. Davis, Z.-Y. Wang, N. Labbe, C.N. Stewart, Jr. 2020. Development and field assessment of transgenic hybrid switchgrass for biofuel traits.  Euphytica 216:25. doi: 10.1007/s10681-020-2558-3

Persad, R., D.N. Reuter, L.T. Dice, M.-A. Nguyen, S.B. Rigoulot, J.S. Layton, M.J. Schmid, M.R. Poindexter, A. Occhialini, C.N. Stewart, Jr., S.C. Lenaghan. 2020. The Q-system as a synthetic transcriptional regulator in plants. Frontiers in Plant Science 11: 245. https://doi.org/10.3389/fpls.2020.00245

Rambani, A., V. Pantalone, S. Yang, J.H. Rice, M. Mazarei, P.R. Arelli, K. Meksem, C.N. Stewart, Jr., T. Hewezi. 2020. Identification of introduced and stably inherited DNA methylation variants in soybean associated with soybean cyst nematode parasitism. New Phytologist 227: 168-184.

Mazarei, M., H. L. Baxter, A. Srivastava, G. LiH. Xie, A. Dumitrache, M. Rodriguez, Jr., J.M. Natzke, J.-Y. Zhang, G. B. Turner, R.W. Sykes, M.F. Davis, M.K. Udvardi, Z.-Y.Wang, B.H. Davison, E. Blancaflor, Y. Tang, C.N. Stewart Jr. 2020. Silencing folylpolyglutamate synthetase1 (FPGS1) in switchgrass (Panicum virgatum L.) improves lignocellulosic biofuel production. Frontiers in Plant Science 11: 843.doi: 10.3389/fpls.2020.00843

Salgotra, R.K., C.N. Stewart, Jr. 2020. Functional markers for precision plant breeding.  International Journal of Molecular Sciences 21: 4792.  doi:10.3390/ijms21134792

Brabazon, H., J.M. DeBruyn, S.C. Lenaghan, F. Li, A.Z. Mundorff, D.W. Steadman, C.N. Stewart, Jr. 2020. Plants to remotely detect human decomposition? Trends in Plant Science. https://doi.org/10.1016/j.tplants.2020.07.013

Kline, L.M., P. Voothuluru, S.C. Lenaghan, J.N. Burris, M. Soliman, L. Tetard, C.N. Stewart, Jr., T.G. Rials, N. Labbé. 2020. A robust method to quantify cell wall bound phenolics in plant suspension culture cells using pyrolysis-gas chromatography/mass spectrometry. Frontiers in Plant Science 11: 574016.  https://doi.org/10.3389/fpls.2020.574016

Guan Z., B.-C. Shen, W. Wei, Z.-X. Tang, C.N. Stewart, Jr. 2020. Loss of C-genome-specific markers during the transgene introgression from Brassica napus to wild B. juncea.  Nordic Journal of Botany. DOI: 10.1111/njb.02794

Li, F, C. Piasecki, R.J. Millwood, B. Wolfe, M. Mazarei, C.N. Stewart, Jr. 2020.  UAV-based high-throughput switchgrass phenotyping and biomass modeling. Frontiers in Plant Science doi: 10.3389/fpls.2020.574073. 

Occhialini, A., A.C. Pfotenhauer, T.P. Frazier, L. Li, S.A. Harbison, A.J. Lail, Z. Mebane, A.A. Piatek, S.B. Rigoulot, H. Daniell, C.N. Stewart, Jr., S.C. Lenaghan. 2020. Generation, analysis, and transformation of macro-chloroplast potato (Solanum tuberosum) lines for chloroplast biotechnology (Solanum tuberosum). Scientific Reports 10: 21144. DOI: 10.1038/s41598-020-78237-x  

2019 Publications


Baxter, H.L., M. Mazarei, A. Dumitrache, J.M. Natzke, M. Rodriguez, Jr., J. Gou, C. Fu, R.W. Sykes, G.B. Turner,M.F. Davis, S.D. Brown, B.H. Davison, Z.-Y. Zhang, C.N. Stewart Jr.2018. Transgenic miR156 switchgrass in the field: growth, recalcitrance and rust susceptibility. Plant Biotechnology Journal 16:39-49.  http://onlinelibrary.wiley.com/doi/10.1111/pbi.12747/full ​

Hewezi, T, V. Pantalone, M. Bennett, C.N. Stewart, Jr., T.M. Burch-Smith. 2018. Phytopathogen-induced changes to plant methylomes. Plant Cell Reports 1: 17-23. https://link.springer.com/article/10.1007/s00299-018-2253-1

Mahas, A., C.N. Stewart, Jr., M.M. Mahfouz. 2018. Harnessing CRISPR/Cas systems for programmable transcriptional and post-transcriptional regulation. Biotechnology Advances  36: 295-310. https://doi.org/10.1016/j.biotechadv.2017.11.008 

Biswal, A.K., M.A. Atmodjo,M. Li,H.L. Baxter, C.G. Yoo, Y. Pu,Y.-C. Lee, M. Mazarei,I.M. Black, J.-Y. Zhang, H. Ramanna, A.L. Bray, Z.R. King, P.R. LaFayette, S. Pattathil, B.S. Donohoe, S.S. Mohanty, D. Ryno, K. Yee, O.A. Thompson, M. Rodriguez, Jr., A. Dumitrache, J. Natzke, K.Winkeler, C. Collins, X. Yang, L. Tan, R.W. Sykes, E.L. Gjersing, A. Ziebell, G.B. Turner, S.R. Decker, M.G.Hahn, B.H. Davison, M.K. Udvardi, J. Mielenz, M.F. Davis, R.S. Nelson, W.A. Parrott, A.J. Ragauskas, C.N. Stewart, Jr., D. Mohnen. 2018. Gains in biomass and biofuel from engineered switchgrass and poplar with downregulated Galacturonosyltransferase (GAUT)4.Nature Biotechnology 36: 249-257. https://www.nature.com/articles/nbt.4067 

Burris, J.N., S.C. Lenaghan, C.N. Stewart, Jr. 2018. Climbing plants: attachment adaptations and bioinspired innovations. Plant Cell Reports 37: 565-574. https://link.springer.com/article/10.1007/s00299-017-2240-y

Xu, W., W. Liu, R. Ye, M. Mazarei, D. Huang, X. Zhang, C.N. Stewart, Jr. 2018. A prolifingene promoter from switchgrass (Panicum virgatum L.) directs strong and specific transgene expression to vascular bundles in rice. Plant Cell Reports 37: 587-597. https://link.springer.com/article/10.1007%2Fs00299-018-2253-1

Liu, W., M. Mazarei, R. Ye, Y. Peng, Y. Shao, H.L. Baxter, R.W. Sykes, G.B. Turner, M.F. Davis, Z.-Y. Wang, R.A. Dixon, C.N. Stewart, Jr. 2018. Switchgrass (Panicum virgatumL.) promoters for green tissue-specific expression of the MYB4transcription factor for reduced-recalcitrance transgenic switchgrass Biotechnology for Biofuels 11:122. https://doi.org/10.1186/s13068-018-1119-7

Piatek, A.A., S.C. Lenaghan, C.N. Stewart, Jr. 2018 Advanced editing of the nuclear and plastid genomes in plants. Plant Science 273: 42-49.  https://europepmc.org/abstract/med/29907308

Stewart, C.N., Jr. R.K. Abudayyeh, S.G. Stewart. 2018. Houseplants as home health monitors. Science 361: 229-330. DOI: 10.1126/science.aau2560. http://science.sciencemag.org/content/361/6399/229

Mazarei, M, H.L. Baxter, M. Li, A.K. Biswal, K. Kim, X.Meng, Y. Pu, W.A. Wuddineh, J.-Y. Zhang, G.B. Turner, R.W. Sykes, M.F. Davis, M.K. Udvardi, Z.-Y. Wang, D. Mohnen, A.J. Ragauskas, N. Labbe, C. N. Stewart Jr.2018. Functional analysis of cellulose synthase CesA4and CesA6genes in switchgrass (Panicumvirgatum) by overexpression and RNAi-mediated gene silencing. Frontiers in Plant Science 9:1114.  doi: 10.3389/fpls.2018.01114. https://www.frontiersin.org/articles/10.3389/fpls.2018.01114/abstract 

Liu, Y., C.N. Stewart, Jr., J. Li, W. Wei. 2018. One species to another: Sympatric Bt-transgene gene flow from Brassica napusalters the reproductive strategy of of wild relative Brassica juncea. Annals of Botany 122: 617-625. DOI: 10.1093/aob/mcy096 
280.  

Schindel, H.S., A.A. Piatek, C.N. Stewart, Jr., S.C. Lenaghan. 2018. The plastid genome as a chassis for synthetic biology-enabled metabolic engineering: players in gene expression. Plant Cell Reports 37: 1419-1429.

Stewart, C.N., Jr. N. Patron, A.D. Hanson, J.M. Jez. 2018. Plant metabolic engineering in the synthetic biology era: plant chassis selection. Plant Cell Reports 37: 1357-1358. 

Zheljazkov, V.D., C.N. Stewart, Jr., B. Joyce, H. Baxter, C.L. Cantrell, T. Astatkie, E. Jeliazkova, C.R. Poovaiah.  2018. Dual utilization of medicinal and aromatic crops for plant chemicals and biofuels. Journal of Agricultural and Food Chemistry 66: 8744-8752. Lenaghan, S.C., Stewart C.N. Jr. 2018 An automated protoplast transformation system. Methods in Molecular Biology vol 1919: page range https://doi.org/10.1007/978-1-4939-8991-1_26​

Holwerda, E.K., R.S. Worthen, N. Kothari, R.C. Lasky, B.H. Davison, C. Fu, Z.-Y. Wang, R.A. Dixon, A.K. Biswal, D. Mohnen, R.S. Nelson, H.L. Baxter, M. Mazarei, C.N. Stewart, Jr., W. Muchero, G.A. Tuskan, C.M. Cai, E.E. Gjersing, M.F. Davis, M.E. Himmel, C.E. Wyman, P. Gilna, L.R. Lynd. 2019. Multiple levers for overcoming the recalcitrance of lignocellulosic biomass.  Biotechnology for Biofuels 12: 15. https://doi.org/10.1186/s13068-019-1353-7

Occhialini, A, A.A. Piatek, A.C. Pfotenhauer, T.P. Frazier, C.N. Stewart, Jr., S.C. Lenaghan. 2019. MoChlo: a versatile modular cloning toolbox for chloroplast biotechnology. Plant Physiology 179: 943-957. DOI: 10.1104/pp.18.01220

Lenaghan, S.C., Stewart C.N. Jr. 2019 An automated protoplast transformation system. Methods in Molecular Biology vol 1919: 355-363. Plant Genome Editing with CRISPR Systems. Humana Press.  

Caverzan, A., C. Piasecki, G. Chavarria, C.N. Stewart, Jr., L. Vargas. 2019. Defenses against ROS in crops and weeds: the effects of interference and herbicides. International Journal of Molecular Sciences 20: 1086.  doi.org/10.3390/ijms20051086

Piasecki, C., Y. Yang, D.P. Benemann, F.S. Kremer, V. Galli, R.J. Millwood, J. Cechin, D. Agostinetto, L.C. Maia, L. Vargas, C.N. Stewart, Jr. 2019 Transcriptomic analysis identifies new non-target site glyphosate-resistance genes in Conyza bonariensis. Plants 8: 157.  doi:10.3390/plants8060157

Liu, Y., C.N. Stewart, Jr. 2019. An exposure-based risk assessment model for GM plants.  Plant Biotechnology Journal 17: 1859-1861. https://doi.org/10.1111/pbi.13146

Pixley, K.V., J. B. Falck-Zepeda, K.E. Giller, L.L. Glenna, F. Gould, C. A. Mallory-Smith, D.M. Stelly, C.N. Stewart, Jr. 2019. Genome editing, gene drives, and synthetic biology: will they contribute to disease-resistant crops, and who will benefit? Annual Review of Phytopathology. 57: 165-188 https://doi.org/10.1146/annurev-phyto-080417-045954

Sultana, M.S., T.P. Frazier, R.J Millwood, S.C. Lenaghan, C.N. Stewart, Jr. 2019. Development and validation of a novel and robust cell culture system in soybean (Glycine max (L.) Merr.) for promoter screening. Plant Cell Reports 38: 1329-1345.    https://doi.org/10.1007/s00299-019-02455-5

Piasecki, C, I.R. Carvalho, J. Cechin, F.A.P. Goulart, L.C. de Maia, D. Agostinetto, A. Caverzan, C.N. Stewart, Jr., L. Vargas. 2019. Oxidative stress and differential antioxidant enzyme activity in glyphosate-resistant and -sensitive hairy fleabane in response to glyphosate treatment. Bragantia 78:379-396.  https://doi.org/10.1590/1678-4499.20180289

Xu, S, S.L. Ottinger, S.M. Schaeffer, J.M. DeBruyn, M. Mazarei, C.N. Stewart, Jr.,  S. Jagadamma, 2019. Effects of field-grown transgenic switchgrass carbon inputs on soil organic carbon cycling. PeerJ 7:e7887. DOI 10.7717/peerj.7887

Ostrov, N., J. Beal, T. Ellis, D.B. Gordon, B.J. Karas, H.H. Lee, S.C. Lenaghan, J.A. Schloss, G. Stracquadanio, A. Trefzer, J.S. Bader, G.M. Church, C.M. Coelho, J.W. Efcavitch, M. Güell,  L.A. Mitchell, A.A.K. Nielsen, B. Peck, A.C. Smith, C.N. Stewart, Jr., H. Tekotte. 2019. Technological challenges and milestones for writing genomes. Science 366: 310-312.   DOI: 10.1126/science.aay0339

One Thousand Plant Transcriptomes Initiative. 2019. One thousand plant transcriptomes and phylogenomics of green plants. Nature 574: 679-685. https://doi.org/10.1038/s41586-019-1693-2  (Cover article).

Piasecki, C, D. Benemann, I.R. Carvalho, D. Agostinetto, C.N. Stewart, Jr., L. Vargas. 2019. Does glyphosate treatment interfere negatively on RNA integrity in glyphosate-resistant and -sensitive Conyza bonariensis?  Planta Daninha 37: e019217909

Liu, W., M.R. Rudis, M.H. Cheplick, R.J. Millwood, J.-P. Yang, C.A. Ondzighi-Assoume, G.A. Montgomery, K.P. Burris, M. Mazarei, J.D. Chesnut, C.N. Stewart, Jr. 2019. Lipofection-mediated genome editing using DNA-free delivery of the Cas9/gRNA ribonucleoprotein into plant cells. Plant Cell Reports 39: 245-257.  https://doi.org/10.1007/s00299-019-02488-w

Lee J.H., M. Mazarei, A.C. Pfotenhauer, A.B. Dorrough, M.R. Poindexter, T. Hewezi, S.C., Lenaghan, D.E. Graham, C.N. Stewart, Jr. 2019. Epigenetic footprints of CRISPR/Cas9-mediated genome editing in plants.  Frontiers in Plant Science 10:1720. doi: 10.3389/fpls.2019.01720

Ondzighi-Assoume, C.A., J.D. Willis, W.K. Ouma, S.M. Allen, Z. King, W.A. Parrott, W. Liu, J.N. Burris, S.C. Lenaghan, C.N. Stewart, Jr. 2019. Embryogenic cell suspensions for high-capacity genetic transformation and regeneration of switchgrass (Panicum virgatum L.). Biotechnology for Biofuels 12:290. DOI : 10.1186/s13068-019-1632-3

2018 Publications


Baxter, H.L., M. Mazarei, A. Dumitrache, J.M. Natzke, M. Rodriguez, Jr., J. Gou, C. Fu, R.W. Sykes, G.B. Turner,M.F. Davis, S.D. Brown, B.H. Davison, Z.-Y. Zhang, C.N. Stewart Jr. 2018. Transgenic miR156 switchgrass in the field: growth, recalcitrance and rust susceptibility. Plant Biotechnology Journal 16:39-49. DOI: 10.1111/pbi.12747/full

Hewezi, T, V. Pantalone, M. Bennett, C.N. Stewart, Jr., T.M. Burch-Smith. 2018. Phytopathogen-induced changes to plant methylomes. Plant Cell Reports 1: 17-23. DOI: 10.1007/s00299-018-2253-1

Mahas, A., C.N. Stewart, Jr., M.M. Mahfouz. 2018. Harnessing CRISPR/Cas systems for programmable transcriptional and post-transcriptional regulation. Biotechnology Advances 36: 295-310 DOI: 10.1016/j.biotechadv.2017.11.008

Biswal, A.K., M.A. Atmodjo, M. Li, H.L. Baxter, C.G. Yoo, Y. Pu, Y.-C. Lee, M. Mazarei, I.M. Black, J.-Y. Zhang, H. Ramanna, A.L. Bray, Z.R. King, P.R. LaFayette, S. Pattathil, B.S. Donohoe, S.S. Mohanty, D. Ryno, K. Yee, O.A. Thompson, M. Rodriguez, Jr., A. Dumitrache, J. Natzke, K.Winkeler, C. Collins, X. Yang, L. Tan, R.W. Sykes, E.L. Gjersing, A. Ziebell, G.B. Turner, S.R. Decker, M.G. Hahn, B.H. Davison, M.K. Udvardi, J. Mielenz, M.F. Davis, R.S. Nelson, W.A. Parrott, A.J. Ragauskas, C.N. Stewart, Jr., D. Mohnen. 2018. Gains in biomass and biofuel from engineered switchgrass and poplar with downregulated Galacturonosyltransferase (GAUT)4. Nature Biotechnology 36: 249-257. DOI: 10.1038/nbt.4067

Burris, J.N., S.C. Lenaghan, C.N. Stewart, Jr. 2018. Climbing plants: attachment adaptations and bioinspired innovations. Plant Cell Reports 37: 565-574. DOI: 10.1007/s00299-017-2240-y

Xu, W., W. Liu, R. Ye, M. Mazarei, D. Huang, X. Zhang, C.N. Stewart, Jr. 2018. A prolifin gene promoter from switchgrass (Panicum virgatum L.) directs strong and specific transgene expression to vascular bundles in rice. Plant Cell Reports 37: 587-597. DOI: 10.1007/s00299-018-2253-1

Liu, W., M. Mazarei, R. Ye, Y. Peng, Y. Shao, H.L. Baxter, R.W. Sykes, G.B. Turner, M.F. Davis, Z.-Y. Wang, R.A. Dixon, C.N. Stewart, Jr. 2018. Switchgrass (Panicum virgatum L.) promoters for green tissue-specific expression of the MYB4 transcription factor for reduced-recalcitrance transgenic switchgrass Biotechnology for Biofuels 11:122. DOI: 10.1186/s13068-018-1119-7 

Piatek, A.A., S.C. Lenaghan, C.N. Stewart, Jr. 2018 Advanced editing of the nuclear and plastid genomes in plants. Plant Science 273: 42-49. DOI: 10.1016/j.plantsci.2018.02.025  

Stewart, C.N., Jr. R.K. Abudayyeh, S.G. Stewart. 2018. Houseplants as home health monitors. Science 361: 229-330. DOI: 10.1126/science.aau2560

Mazarei, M, H.L. Baxter, M. Li, A.K. Biswal, K. Kim, X.Meng, Y. Pu, W.A. Wuddineh, J.-Y. Zhang, G.B. Turner, R.W. Sykes, M.F. Davis, M.K. Udvardi, Z.-Y. Wang, D. Mohnen, A.J. Ragauskas, N. Labbe, C. N. Stewart Jr. 2018. Functional analysis of cellulose synthase CesA4 and CesA6 genes in switchgrass (Panicum virgatum) by overexpression and RNAi-mediated gene silencing. Frontiers in Plant Science 9:1114.  DOI: 10.3389/fpls.2018.01114

Liu, Y., C.N. Stewart, Jr., J. Li, W. Wei. 2018. One species to another: Sympatric Bt-transgene gene flow from Brassica napus alters the reproductive strategy of wild relative Brassica juncea. Annals of Botany 122: 617-625. DOI: 10.1093/aob/mcy096

Schindel, H.S., A.A. Piatek, C.N. Stewart, Jr., S.C. Lenaghan. 2018. The plastid genome as a chassis for synthetic biology-enabled metabolic engineering: players in gene expression. Plant Cell Reports 37: 1419-1429.  DOI: 10.1007/s00299-018-2323-4

Stewart, C.N., Jr. N. Patron, A.D. Hanson, J.M. Jez. 2018. Plant metabolic engineering in the synthetic biology era: plant chassis selection. Plant Cell Reports 37: 1357-1358.

Zheljazkov, V.D., C.N. Stewart, Jr., B. Joyce, H. Baxter, C.L. Cantrell, T. Astatkie, E. Jeliazkova, C.R. Poovaiah.  2018. Dual utilization of medicinal and aromatic crops for plant chemicals and biofuels. Journal of Agricultural and Food Chemistry 66: 8744-8752.

2017 Publications


Johnson, C.R., R.J. Millwood, Y. Tang, J. Gou, R.W. Sykes, G.B. Turner, M.F. Davis, Y. Sang, Z.-Y. Wang, C.N. Stewart, Jr. 2017. Field-grown miR156-transgenic switchgrass reproduction, yield, global gene expression analysis, and bioconfinememt. Biotechnology for Biofuels. DOI :10.1186/s13068-017-0939-1.https://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-017-0939-1

Willis, J.D., J.N. Grant, M. Mazarei, L.M. Kline, C.S Rempe, A.G. Collins, G.B. Turner, S.R. Decker, R.W. Sykes, M.F. Davis, N. Labbe, J.L. Jurat-Fuentes, C.N. Stewart, Jr., 2017. The TcEG1 beetle (Tribolium castaneum) cellulase produced in transgenic switchgrass is active at alkaline pH and auto-hydrolyzes biomass for increased cellobiose release. Biotechnology for Biofuels 10:230. DOI :10.1186/s13068-017-0918-6 https://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-017-0918-6

Burris, J.N., S.C. Lenaghan, C.N. Stewart, Jr. Climbing plants: adaptations and bioinspired innovations. Plant Cell Reports. https://link.springer.com/article/10.1007/s00299-017-2240-y

Nelson, R.S., C.N. Stewart, Jr., J. Gou, S. Holladay, L. Gallego-Giraldo, A. Flanagan, D.G.J. Mann, H. Hisano, W.A. Wuddineh, C.R. Pooviaiah, A. Srivastava, A.K. Biswal, H. Shen, L.L. Escamilla-Treviño, J. Yang, C.F. Hardin, R. Nandakumar,C. Fu, J. Zhang, X. Xiao, R. Percifield, F. Chen, J.L. Bennetzen, M. Udvardi, M. Mazarei, R.A. Dixon, Z.-Y. Wang, Y. Tang, D. Mohnen, B. H. Davison. 2017. Development and use of a switchgrass (Panicum virgatum L.) transformation pipeline by the BioEnergy Science Center to evaluate plants for reduced cell wall recalcitrance. Biotechnology for Biofuels 10:309 https://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-017-0991-x

Johnson, C.R., R.J. Millwood, Z.-Y. Wang, C.N. Stewart, Jr. 2017. Light and temperature effects on miR156 transgenic switchgrass flowering: a simulated latitudinal study. Plant Direct 2017; 1:1-6. http://onlinelibrary.wiley.com/doi/10.1002/pld3.26/pdf  

Millwood, R., M. Naghugiri-Rao, R. Ye, E. Terry-Emert, C.R. Johnson, M. Hanson, J.N. Burris, C. Kwit, C.N. Stewart, Jr. 2017. Pollen-mediated gene flow from transgenic to non-transgenic switchgrass (Panicum virgatum L.) in the field. BMC Biotechnology 17:40. https://bmcbiotechnol.biomedcentral.com/articles/10.1186/s12896-017-0363-4

Grant, J.N., J.N. Burris, C.N. Stewart, Jr., S.C. Lenaghan. 2017.  Improved tissue culture conditions for the emerging C4 model Panicum hallii. BMC Biotechnology 17:39. https://bmcbiotechnol.biomedcentral.com/articles/10.1186/s12896-017-0359-0

Gould, F., R.M. Amasino, D. Brossard, C.R. Buell, R.A. Dixon, J.B. Falk-Zepeda, M.A. Gallo, K.E. Giller, L.L. Glenna, T. Griffin, B.R. Hamaker, P.M. Kareiva, D. Magraw, C. Mallory-Smith, K.V. Pixley, E.P. Ransom, M. Rodemeyer, D.M. Stelley, C.N. Stewart, Jr. R.J. Whitaker. 2017. Elevating the conversation about GE crops. Nature Biotechnology 35: 302-304. https://www.nature.com/articles/nbt.3841

Stewart, C.N., Jr. 2017. Becoming weeds. Nature Genetics 49:654-655. http://www.nature.com/ng/journal/v49/n5/full/ng.3851.html 

Stewart, C.N., Jr.W. Liu. 2017. Synthetic promoters for precise control of gene expression in plants. Chemical Engineering Progress 113: 36-39. https://www.aiche.org/resources/publications/cep/2017/april/synthetic-promoters-precise-control-gene-expression-plants

Rempe, C.S., K.P Burris, S.C. Lenaghan, C.N. Stewart, Jr. 2017. The potential of systems biology to discover antibacterial mechanisms of plant phenolic. Frontiers in Microbiology 8:422. http://trace.tennessee.edu/cgi/viewcontent.cgi?article=1087&context=utk_planpubs  

Dumitrache, A., J. Natzke, M. Rodriguez, Jr., K.L. Yee, O.A. Thompson, C.R. Poovaiah, H. Shen,M. Mazarei, H.L. Baxter, C. Fu, Z.-Y. Wang, A.K. Biswal, G. Li, A.C. Srivastava, Y. Tang, C.N. Stewart, Jr., R.A. Dixon, R.S. Nelson, D. Mohnen, J. Mielenz, S.D. Brown, B.H. Davison. 2017. Transgenic switchgrass (Panicum virgatum L.) targeted for reduced recalcitrance to bioconversion: a 2-year comparative analysis of field-grown lines modified for target gene or genetic element expression. Plant Biotechnology Journal 15: 688-697. http://onlinelibrary.wiley.com/doi/10.1111/pbi.12666/full 

Rempe, C.S., S.C. Lenaghan, K.P. Burris, C.N. Stewart, Jr. 2017.Metabolomic analysis of the mechanism of action of yerba mate aqueous extract on Salmonella enterica serovar Typhimurium. Metabolomics 13:16. https://link.springer.com/article/10.1007/s11306-016-1159-6

DeBruyn, J. M.; D.A. Bevard, M.E. Essington, J.Y. McKnight, S.M. Schaeffer, H.L. Baxter, M. Mazarei, D.G.J. Mann,  R.A. Dixon, F. Chen, C Zhuo, Z.-Y. Wang, C.N. Stewart, Jr. 2017.  Field grown transgenic switchgrass (Panicum virgatum L.) with altered lignin does not affect soil chemistr y, microbiology and carbon storage potential. GCB Bioenergy 9: 1100-1109. http://onlinelibrary.wiley.com/doi/10.1111/gcbb.12407/full

Li, M., Y. Pu, C.G. Yoo, E. Gjersing, S.R. Decker, C. Doeppke, T. Shollenberger, T.J. Tschaplinski, N.L. Engle, R.W. Sykes, M.F. Davis, H.L. Baxter, M. Mazarei, C. Fu, R.A. Dixon, Z.-W. Wang, C.N. Stewart, Jr., A.J. Ragauskas. 2017. Study of traits and recalcitrance reduction of field-grown COMT down-regulated switchgrass. Biotechnology for Biofuels 10: 12. https://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-016-0695-7

Lin, J., D. Wang, X. Chen. T.G. Köllner,  M. Mazarei, H. Guo, V.R. Pantalone, P. Arelli, C.N. Stewart, Jr., N. Wang, F. Chen. 2017. An (E,E)-α-farnesene synthase gene of soybean has a role in defence against nematodes and is involved in synthesizing insect-induced volatiles. Plant Biotechnology Journal 15: 510-519.http://onlinelibrary.wiley.com/doi/10.1111/pbi.12649/full 

2016 Publications


Dlugosz, E.M., S.C. Lenaghan, C.N. Stewart, Jr. 2016.  A robotic platform for high-throughput protoplast isolation and transformation. Journal of Visualized Experiments (115), e54300, doi: 10.3791/54300http://www.jove.com/video/54300/a-robotic-platform-for-high-throughput-protoplast-isolation

Direct link to download video: A Robotic Platform for High-throughput Protoplast Isolation and Transformation

Willis, J.D., J.A. Smith, M. Mazarei, J.-Y. Zhang, G.B. Turner, S.R. Decker, R.W. Sykes, C.R. Poovaiah, H.L. Baxter, M.F. Davis, M.K. Udvardi, M.J. Peña, J. Backe, M. Bar-Peled, C. N. Stewart, Jr. 2016. Downregulation of a UDP-arabinomutase gene in switchgrass (Panicum virgatum L.) results in increased cell wall lignin while reducing arabinose-glycans. Frontiers in Plant Science 7:1580. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5081414/

Lin, J. M. Mazarei, N. Zhao, C.A. Hatcher, W.A. Wuddineh, M. Rudis, T.J. Tschaplinski, V.R. Pantalone, P.R. Arelli, T. Hewezi, F. Chen, C.N. Stewart, Jr. 2016. Transgenic soybeanoverexpressing GmSAMT1 exhibits resistance to multiple-HG types of soybean cyst nematode Heterodera glycines. Plant Biotechnology Journal 14: 2100-2109. http://onlinelibrary.wiley.com/doi/10.1111/pbi.12566/full

Pigna, G., T. Dhillon, E.M. Dlugosz, J.S. Yuan, C. Gorman, P. Morandini, S.C. Lenaghan, C.N. Stewart, Jr.  2016. Methods for suspension culture, protoplast extraction, and transformation of high-biomass yielding perennial grass Arundo donax . Biotechnology Journal 11: 1657-1666.

Correction: http://onlinelibrary.wiley.com/doi/10.1002/biot.201770023/full

Lane, T.S., C.S. Rempe, J. Davitt, M.E.Staton, Y. Peng, D.E. Soltis, M. Melkonian, M. Deyholos, J.H. Leebens-Mack, M. Chase, C.J. Rothfels, D. Stevenson, S.W Graham, J.Yu, T. Liu, J.C. Pires, P.P. Edgar, Y. Zhang, Y. Xie, Y. Zhu, E. Carpenter, G.K.-S. Wong, C.N. Stewart, Jr. 2016. Diversity of ABC transporter genes across the plant kingdom and their potential utility in biotechnology. BMC Biotechnology 16:47. http://www.biomedcentral.com/1472-6750/16/47

Willis, J.D., M. Mazarei, C.N. Stewart, Jr. 2016. Transgenic plant-produced hydrolytic enzymes and the potential of insect gut-derived hydrolases for biofuels. Frontiers in Plant Science 7: 765. http://journal.frontiersin.org/article/10.3389/fpls.2016.00675/abstract

Cardi, T., C.N. Stewart, Jr. 2016. Progress of targeted genome modification approaches in higher plants. Plant Cell Reports 35: 1401-1416.

Altpeter, F., N.M. Springer, L.E. Bartley, A.E. Blechl, T.P. Brutnell, V. Citovsky, L.J. Conrad, S.B. Gelvin, D.P. Jackson, A.P. Kausch, P.G. Lemaux, J.I. Medford, M.L. Orozco-Cárdenas, D.M. Tricoli, J. Van Eck,  D.F. Voytas, V. Walbot, K. Wang, Z.J. Zhang, C.N. Stewart, Jr. 2016. Advancing crop transformation in the era of genome editing.  Plant Cell 15:1510-1520 doi: http:/ / dx. doi. org/ 10. 1105/ tpc. 16. 00196

Liu, W., C.N. Stewart, Jr. 2016. Plant synthetic promoters and transcription factors. Current Opinion in Biotechnology 37: 36-44.
Burris, K.P., E.M Dlugosz, A.G. Collins, C.N. Stewart, Jr., S.C. Lenaghan. 2016. Development of a rapid, low-cost protoplast transfection system for switchgrass (Panicum virgatum L.). Plant Cell Reports 35: 693-704.

Millwood, R.J., H.S. Moon, C.R. Poovaiah, B. Muthukumar, J.H. Rice. J.M. Abercrombie, L.L Abercrombie, W.D. Green, C.N. Stewart, Jr. 2016. Engineered selective plant male sterility through pollen-specific expression of the EcoRI restriction endonuclease. Plant Biotechnology Journal 14: 1281-1290.  http://onlinelibrary.wiley.com/doi/10.1111/pbi.12493/epdf

Wuddineh, W.A., M. Mazarei, J.-Y. Zhang, G. Turner, R. Sykes, S.R. Decker, M.F. Davis, M. Udvardi, C.N. Stewart, Jr. 2016. Identification and overexpression of a Knotted1-like transcription factor in switchgrass (Panicum virgatum L.) for lignocellulosic feedstock improvement. Frontiers in Plant Science 7:520.  http://journal.frontiersin.org/article/10.3389/fpls.2016.00520/abstract

Baxter, H.L.,M. Mazarei, C. Fu, K. Cheng, G.B. Turner,R.W. Sykes, M.T. Windham, M.F. Davis, R.A. Dixon, Z.-Y.Wang, C.N. Stewart, Jr. 2016. Time course field analysis of COMT-downregulated switchgrass: lignification, recalcitrance, and rust susceptibility. BioEnergy Research 9: 1087-1100. http://bioenergycenter.org/besc/publications/baxter_time_yr9.pdf

Manshardt, R., D. Bishaw, K. Pitz, C.N. Stewart 2016. Gene flow from commercial transgenic papaya fields into feral populations in Hawaii. Acta Horticulturae. 1124: 33-40
https://doi.org/10.17660/ActaHortic.2016.1124.5  

2015 Publications 


Baxter, H.L., L.W. Alexander, M. Mazarei, E. Haynes, G.B. Turner, R.W. Sykes, S.R. Decker, M.F. Davis, R.A. Dixon, Z.-Y.Wang, C.N. Stewart, Jr.  2016. Hybridization of downregulated-COMT transgenic switchgrass lines with field selected switchgrass for improved biomass traits. Euphytica 209: 341-355.

Wuddineh, W.A., M. Mazarei, G.B. Turner, R.W. Sykes, S.R. Decker, M.F. Davis, C.N. Stewart, Jr. 2015 Identification and molecular characterization of the switchgrass AP2/ERF transcription factor superfamily, and overexpression of PvERF001 for improvement of biomass characteristics for biofuel. Frontiers in Bioengineering and Biotechnology 3: 101.  http://journal.frontiersin.org/article/10.3389/fbioe.2015.00101/abstract

Baxter, H.L., C.R. Poovaiah, K.L. Yee, M. Mazarei, M. Rodriguez, Jr., O.A. Thompson, H. Shen, G.B. Turner, S.R. Decker, R.W. Sykes, F. Chen, M.F. Davis, J.R. Mielenz, B.H. Davison, R.A. Dixon, C.N. Stewart Jr. 2015. Field evaluation of transgenic switchgrass plants overexpressing PvMYB4 for reduced biomass recalcitrance. BioEnergy Research 8:910-921.

Joyce, B.L., V.D. Zheljazkov, R. Sykes, C.L. Cantrell, C. Hamilton, D.G.J. Mann, M. Rodriguez, J.R. Mielenz, T. Astatkie, and C.N. Stewart, Jr. 2015. Ethanol and high-value terpene co-production from lignocellulosic biomass of Cymbopogon flexuosus and Cymbopogon martinii. PLoS ONE 10(10): e0139195.  http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0139195

Liu, Y., Y.-X. Zhang, S.-Q. Song, J. Li, C.N. Stewart, Jr., W. Wei, Y. Zhao, W.-Q. Wang.  2015. A proteomic analysis of seeds from Bt-transgenic Brassica napus and hybrids with wild B.juncea. Scientific Reports 5: 15480. http://www.nature.com/articles/srep15480

Liu, Y., C. N. Stewart, Jr., J. Li, H.  Huang, X.  Zhang. 2015.The presence of Bt-transgenic oilseed rape in wild mustard populations affects plant growth. Transgenic Research 24: 1043-1053.

Rempe, C.S., K.P. Burris, H.L. Woo, B. Goodrich, D.K. Gosnell, T.J. Tschaplinski, C.N. Stewart, Jr. 2015. Computational ranking of yerba mate small molecules based on their predicted contribution to antibacterial activity against methicillin-resistant Staphylococcus aureus. PLOS ONE  10(5): e0123925.  http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0123925

Wuddineh, W.A., M. Mazarei, J. Zhang, C.R. Poovaiah, D.G.J. Mann, A. Ziebell, R.W. Sykes, M.F. Davis, M.K. Udvardi, C.N. Stewart, Jr. 2015. Identification and overexpression of gibberellin 2-oxidase (GA2ox) in switchgrass (Panicum virgatum L.) for improved plant architecture and reduced biomass recalcitrance.  Plant Biotechnology Journal 13: 636-647.

Liu, Y.-B., H. Darmency, C. N. Stewart, Jr., W. Wei, Z.-X. Tang, K.-P. Ma. 2015. The effect of Bt-transgene introgression on plant growth and reproduction in wild Brassica juncea. Transgenic Research 24: 537-547.

Liu, J., N. Nana, J.N. Grant, Z.-M. Cheng, C.N. Stewart, Jr., T. Hewezi. 2015.  Soybean kinome: functional classification and gene expression patterns. Journal of Experimental Botany 66: 1919-1934.

Cannon, S.B., M.R. McKain, A. Harkess, M.N. Nelson, S. Dash, M.K. Deyholos, Y. Peng, B. Joyce, C.N. Stewart, Jr., M. Rolf, T. Kutchan, X. Tan, C. Chen, Y. Zhang, E. Carpenter, G.K.-S. Wong, J.J. Doyle, J. Leebens-Mack. 2015. Multiple polyploidy events in the early radiation of nodulating and nonnodulating legumes. Molecular Biology and Evolution 32: 193-210.

Trumbo, J.L., B. Zhang, and C.N. Stewart, Jr. 2015. Manipulating microRNAs for improved biomass and biofuels from plant feedstocks. Plant Biotechnology Journal 13: 337-354.

Burris, K.P., K.L. Higginbotham, C.N. Stewart, Jr.  2015. Aqueous extracts of yerba mate as bacteriacidal agents against methicillin-resistant Staphylococcus aureus in a microbiological medium and ground beef mixtures. Food Control 50: 748-753.

Okada, M.,  B.D. Hanson, K.J. Hembree, Y. Peng, A. Shrestha, C.N. Stewart, Jr., S.D. Wright, and M. Jasieniuk. 2015.  Evolution and spread of glyphosate resistance in Conyza bonariensisin California and a comparison with closely related Conyza canadensis. Weed Research 55:173-184.

Poovaiah, C.R., M. Mazarei, S.R. Decker, G.B. Turner, R.W. Sykes, M.F. Davis, C.N. Stewart, Jr.2015. Overexpression of switchgrass sucrose synthase (PvSUS1) increases plant biomass in transgenic switchgrass (Panicum virgatum L.) . Biotechnology Journal 10: 552-563.  

Liu, W., and C.N. Stewart, Jr. Plant synthetic biology. 2015 Trends in Plant Science 20: 309-317.

Piya, S., S.K. Shrestha, B. Binder, C.N. Stewart, Jr., T. Hewezi.  2015. Protein-protein interaction and gene co-expression maps of ARFs and Aux/IAAs in Arabidopsis. Frontiers in Plant Science 5: 744 (http://journal.frontiersin.org/article/10.3389/fpls.2014.00744/abstract)  

Liu, Y. C.N. Stewart, Jr., J. Li. 2015. China: inform public on GM, don’t cheerlead. Nature 520: 295. 

2014 Publications


Alexander, L.W., E.R. Haynes, J. Burris, S. Jackson, C.N. Stewart, Jr. 2014. Cultural treatments for accelerated growth and flowering of Panicum virgatum Biofuels 5:771-780

Peng, Y., Z. Lai, T. Lane, M. Nageswara-Rao, M. Okada, M. Jasieniuk, H. O’Geen, R.W. Kim, R.D. Sammons, L. Rieseberg, C.N. Stewart, Jr. 2014. De novo genome assembly of the economically-important weed Conyza canadensis using integrated data from multiple sequencing platforms. Plant Physiology 166: 1241-1254. 
http://www.plantphysiology.org/content/166/3/1241.full.pdf+html?sid=262cf1b9-a53a-4117-92f0-cf45625637e1

Ji, H., Y. Peng, N. Meckes, S. Allen, C.N. Stewart, Jr., and M.B. Traw. 2014. ATP-dependent binding cassette transporter G family member 16 increases plant tolerance to abscisic acid and assists in basal resistance against Pseudomonas syringae DC3000. Plant Physiology 166: 878-888.   
http://www.plantphysiology.org/content/166/2/879.full.pdf+html?sid=ffaba094-f046-47ee-bdb7-7124271ffb5f

Joyce, B.L., S. Eda, J. Dunlap, and C.N. Stewart, Jr. 2014. Morphology and ploidy level determination of Pteris vittata callus during induction and regeneration. BMC Biotechnology 14:96. http://www.biomedcentral.com/1472-6750/14/96/

Poovaiah, C., M. Nagaswara-Rao, J.R. Soneji, H.L. Baxter, C.N. Stewart, Jr. 2014. Altered lignin  biosynthesis using biotechnology to improve lignocellulosic biofuel feedstocks. Plant Biotechnology Journal 12: 1163-1173. 

Peng, Y., S. Allen, R.J. Millwood, C.N. Stewart, Jr. 2014.  Building the ‘Fukusensor:’ a genetically engineered plant for reporting DNA damage in response to gamma radiation. Plant Biotechnology Journal 12:1329-1332.

Floyd, S.K., J.G. Ryan,  S.J. Conway, E. Brenner, K.P. Burris, J.N. Burris, T. Chen, P.P. Edger, S.W. Graham, J.H. Leebens-Mack, J.C. Pires, C.J. Rothfels, E.M. Sigel, D.W. Stevenson, C.N. Stewart, Jr., G.K.-S. Wong, J.L. Bowman. 2014. Origin of a novel regulatory module by duplication and degeneration of an ancient plant transcription factor. Molecular Phylogenetics and Evolution 81: 159-173.

Chauhan, A. A. Smartt, J.Wang, S. Utturkar, A. Frank, M. Bi, J. Liu, D. Williams, T. Xu, M. Eldridge, A. Arreaza, A. Rogers, H.C. Gonzalez, A.C. Layton, H.L. Baxter, M. Mazarei, J.M. DeBruyn, C.N. Stewart, Jr., S.D. Brown, L.J. Hauser, G.S. Sayler.  2014. Integrated metagenomics and metatranscriptomics analyses of root-associated soil from transgenic switchgrass. Genome Announcements 2:e00777-14. doi:10.1128/genomeA.00777-14.    
http://genomea.asm.org/content/2/4/e00777-14.full

Nageswara-Rao, M., M. Hanson, S. Agarwal, C.N. Stewart Jr. C. Kwit. 2014. Genetic diversity analysis of switchgrass (Panicum virgatum L.) populations using microsatellites and chloroplast sequences. Agroforestry Systems 88: 823-834.

Mahfouz, M.M., A. Piatek, C.N. Stewart Jr. Genome engineering via TALENs and CRISPR/Cas9 systems: challenges and perspectives. Plant Biotechnology Journal 12: 1006-1014.

Liu, W., M. Mazarei, Y. Peng, M.H. Fethe, M.R. Rudis, J. Lin, R.J. Millwood, P.R. Arelli, C.N. Stewart, Jr. 2014. Computational discovery of soybean promoter cis-regulatory elements for the construction of soybean cyst nematode-inducible synthetic promoters. Plant Biotechnology Journal 12: 1015-1026

Baxter, H.L, M. Mazarei, N. Labbe, L. Kline, Q. Cheng, M. Windham, D.G.J. Mann, C. Fu, A. Ziebell, R. Sykes, M. Rodriguez, M. Davis, J. Mielenz, R.A. Dixon, Z.-Y. Wang, C.N. Stewart, Jr. 2014. Two-year field analysis of decreased recalcitrance transgenic switchgrass. Plant Biotechnology Journal 12: 914-924 (with accompanying journal cover).

Fethe, M.H. W. Liu, J.N. Burris, R.J. Millwood, M. Mazarei, M.R. Rudis, D.G. Yeaman, M. Dubosquielle, C.N. Stewart Jr. 2014 The performance of pathogenic bacterial phytosensing transgenic tobacco in the field. Plant Biotechnology Journal 12: 755-764.

Ali, A., J.C. Zinnert, B. Muthukumar, Y. Peng, S.-M. Chung, C.N. Stewart, Jr. 2014. Physiological and transcriptional responses of Baccharis halimifolia to the explosive ‘composition B’ (RDX/TNT) in amended soil. Environmental Science and Pollution Research 21: 8261-8270.
Cao, D., C.N. Stewart Jr, M. Zheng, Z. Guan, Z.-X. Tang, W. Wei, K.-P. Ma. 2014. Stable Bacillus thuringiensis transgene introgression from Brassica napus to wild mustard B. juncea. Plant Science 227: 45-50.

Salgotra, R.K., B.B. Gupta, C.N. Stewart, Jr. 2014. From genomics to functional markers in the era of next-generation sequencing. Biotechnology Letters 36: 417-426. 

Liu, W., M.R. Rudis, Y. Peng, M. Mazarei, R.J. Millwood, J.-P. Yang, W. Yu, J.D. Chesnut,  C.N. Stewart Jr. 2014. Synthetic TAL  effectors for targeted enhancement of transgene expression in plants. Plant Biotechnology Journal 12: 436-446 (with accompanying journal cover).

Xie, F., C.N. Stewart, Jr., F. Taten, Q. He, H. Liu, B. Zhang. 2014. High throughput deep sequencing shows that microRNAs play important roles in switchgrass responses to drought and salinity stress for biofuel production. Plant Biotechnology Journal12: 354-366.

Gressel, J., C.N Stewart, Jr., L.V. Giddings, A.J. Fischer, J.C. Streibig, N.R. Burgos, A. Trewavas, A. Merotto, Jr., C.J. Leaver, K. Ammann, V. Moses, A. Lawton-Rauh. 2014. Overexpression of epsps transgene in weedy rice: insufficient evidence to support multiple conclusions about biosafety.  New Phytologist 202:360-362.

Resnick, D.B., and C.N. Stewart, Jr. 2014. Expanding the scope of responsible conduct in research instruction. Accountability in Research 21:321-327.
Higginbotham, K.L., K.P. Burris, S. Zivanovic, P.M. Davidson, C.N. Stewart, Jr. 2014.Antimicrobial activity of Hibiscus sabdariffa aqueousextracts against Escherichia coli O157:H7 and Staphylococcus aureus in a microbiological medium and milk of varying fat concentrations. Journal of Food Protection 77: 362-368.

Higginbotham, K.L., K.P. Burris, S. Zivanovic, P.M. Davidson, C.N. Stewart, Jr. 2014. Aqueous extracts of Hibiscus sabdariffa calyces as an antimicrobial rinse on hot dogs against Listeria monocytogenes and methicillin-resistant Staphylococcus aureusFood Control 40: 274-277.

2013 Publications


Shen H., M. Mazarei, H. Hisano, F. Hardin, L.L. Escamilla-Treviño, C. Fu, Y. Pu, M.R. Rudis, Y. Tang, X. Xiao, L. Jackson, G. Li, T. Hernandez, F. Chen, A.J. Ragauskas, C.N. Stewart Jr., Z.-Y. Wang, and R.A. Dixon. 2013. A genomics approach to deciphering lignin biosynthesis in switchgrass (Panicum virgatum L.) .  Plant Cell 25: 4342-4361.

Lin, J. M. Mazarei, N. Zhao, J.J. Zhu, X Zhaung, W. Liu., V.R. Pantalone, P.R. Arelli, C. N. Stewart, Jr.,  F. Chen. 2013.  Overexpression of a soybean salicyclic acid methlyltransferase confers resistance to soybean cyst nematode. Plant Biotechnology Journal 11: 1135-1145.

Ellstrand, N.C., P. Meirmans, J. Rong, D. Bartsch, A. Ghosh, T.J. de Jong, P. Haccou, B.-R. Lu, A.A. Snow, C.N. Stewart, Jr., J.L. Strasburg, P.H. van Tienderen, K. Vrieling, D. Hooftman. 2013. Introgression of crop alleles into wild or weedy populations. Annual Review of Ecology, Evolution, and Systematics, 44: 325-345.

Baxter, H.L. and C.N. Stewart, Jr. 2013. Effects of altered lignin biosynthesis on phenylpropanoid metabolism and plant stress.  Biofuels 4: 635-650.

Liu, W., J.S. Yuan and C.N. Stewart, Jr. 2013. Advanced genetic tools for plant biotechnology. Nature Reviews Genetics 14: 781-793.      Supplement. Rice, J.H., R.E. Mundell, R.J. Millwood, O.D. Chambers, L.G. Abercrombie, H.M. Davies, and C.N. Stewart. Jr. 2013.  An orange fluorescent protein tagging system for real-time pollen tracking. BMC Research Notes 6:383. http://www.biomedcentral.com/1756-0500/6/383/abstract

Muthukumar, B., B.L. Joyce, M.P.  Elless, C.N. Stewart Jr. 2013. Biolistics- and Agrobacterium tumefaciens-mediated  stable transformation of Pteris vittta (Chinese brake fern) and Ceratopteris thalictroides (C-fern) spores. Plant Physiology 163: 648-658.

Nagaswara-Rao, M., J. Soneji, C. Kwit, C.N. Stewart, Jr. 2013. Advances in biotechnology and genomics of switchgrass. Biotechnology for Biofuels, 6:77. http://www.biotechnologyforbiofuels.com/content/6/1/77/abstract

Rice, J.H., R.E. Mundell, R.J. Millwood, O.D. Chambers, C.N. Stewart. Jr. and H.M. Davies, 2013.  Assessing the bioconfinement potential of a Nicotiana hybrid platform for use in plant molecular farming applications. BMC Biotechnology, 13:63. http://www.biomedcentral.com/1472-6750/13/63

Nageswara-Rao, M., C. Kwit, S. Agarwal, M.T. Patton, J.A. Skeen, J.S. Yuan, R.M. Manshardt. C.N. Stewart Jr. 2013. Sensitivity of a real-time PCR method for the detection of transgenes in a mixture of transgenic and non-transgenic seeds of papaya (Carica papaya L.) BMC Biotechnology, 13:69. http://www.biomedcentral.com/1472-6750/13/69

Burris, K.P., T-C. Wu, M. Vasudev, M.A. Stroscio, R.J. Millwood, and C.N. Stewart, Jr., 2013. Mega-nano detection of foodborne pathogens and transgenes using molecular beacon and semiconductor quantum dot technologies.  IEEE Transactions in NanoBioscience, 12: 233-238.

Okada, M., B.D. Hanson, K.J. Hembree, W.T. Lanini, Y. Peng, A. Shrestha, C.N. Stewart, Jr., S.D. Wright, and M. Jasieniuk. 2013 Evolution and spread of glyphosate resistance in Conyza canadensis in California. Evolutionary Applications, 6: 761-777.

Lenaghan, S., J.N. Burris,  K. Chourey, Y. Huang, L. Xia, B. Lady, R. Sharma, C. Pan, Z. LeJeune, S. Foister, R.L. Hettich,  C.N. Stewart, Jr. and M. Zhang. 2013.  Identification of the chemical nature of nanoparticles secreted by English ivy (Hedera helix). Journal of the Royal Society Interface, 10 (87): 20130392.

Hardin, C.F. C. Fu, H. Hisano, X. Xaio, H. Shen, C.N. Stewart, Jr., W. Parrott, R.A. Dixon, Z-Y. Wang. 2013. Standardization of switchgrass sample collection for cell wall and biomass trait analysis. BioEnergy Research, 6: 755-762. http://link.springer.com/content/pdf/10.1007%2Fs12155-012-9292-1.pdf

Huang, Y., S.C. Lenaghan, L. Xia, Z. Xu, J.N. Burris, C.N. Stewart Jr., M.J. Zhang. 2013. Characterization of physicochemical properties of ivy nanoparticles for cosmetic applications. Journal of Nanobiotechnology, 11:3. http://www.jnanobiotechnology.com/content/11/1/3

Shen, H., C. R. Poovaiah, A. Ziebell, T.J. Tschaplinski, S. Pattathil,  E. Gjersing, N. Engle,  R. Katahira, Y. Pu, R. Sykes, F. Chen, A.J. Ragauskas, J.R. Mielenz, M.G. Hahn, M. Davis, C.N. Stewart, Jr., R.A. Dixon. 2013 Enhanced characteristics of genetically modified switchgrass (Panicum virgatum L.) for high biofuel production. Biotechnology for Biofuels, 6:71. http://www.biotechnologyforbiofuels.com/content/6/1/71/abstract

Sang, Y., R.J. Millwood, C.N. Stewart Jr. 2013 Gene use restriction technologies for transgenic plant bioconfinement. Plant Biotechnology Journal, 11:649-658.

Nagaswara-Rao, M., J. Soneji, C. Kwit, C.N. Stewart, Jr. 2013. Advances in biotechnology and genomics of switchgrass. Biotechnology for Biofuels, 6:77. http://www.biotechnologyforbiofuels.com/content/6/1/77/abstract

Liu, W., M. Mazarei, M.R. Rudis, M.H. Fethe, Y. Peng, R.J. Millwood, G. Schoene, J.N. Burris and C.N. Stewart, Jr. 2013. Bacterial pathogen phytosensing in transgenic tobacco and Arabidopsis plants. Plant Biotechnology Journal, 11:43-52.

Yau, Y.Y. and C.N. Stewart, Jr. 2013. Less is more: strategies to remove marker genes from transgenic plants. BMC Biotechnology, 13:36. http://www.biomedcentral.com/1472-6750/13/36

Nageswara-Rao, M., C.N. Stewart, Jr., C. Kwit. 2013. Genetic diversity and structure of natural and agronomic switchgrass (Panicum virgatum L.) populations. Genetic Resources and Crop Evolution, 60: 1057-1068. http://bioenergycenter.org/besc/publications/nageswara_rao_genetic_yr5.pdf

2012 Publications


Burris, J.N., S. Lenaghan, M.J. Zhang., C.N. Stewart, Jr. 2012.  Nanoparticle biofabrication using English ivy (Hedera helix). Journal of Nanobiotechnology, 10:41. http://www.jnanobiotechnology.com/content/10/1/41 

Johnson, MTJ, Z Tian, EJ Carpenter, R Bruskiewich, JN Burris, CT Carrigan, MW Chase, ND Clarke, S Covshoff, CW dePamphilis, PP Edger, F Goh, S Graham, S Greiner, JM Hibberd, I Jordon-Thaden, TM Kutchan, J Leebens-Mack, M Melkonian, N Miles, H Myburg, JC Pires, M Rolf, RF Sage, D Soltis, P Soltis, D Stevenson, CN Stewart, Jr, B.Surek, CJM Thomsen, JC Villarreal, X Wu, Y Zhang, MK Deyholos & GK-S Wong. 2012. Evaluating methods of isolating total RNA and predicting the success of sequencing phylogenetically diverse plant transcriptomes. PLoS One, 11:e50226. http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0050226

Nageswara Rao M,Stewart Jr. CN, Kwit C. 2012. Genetic diversity and structure of natural and cultivated switchgrass (Panicum virgatum L.) populations. Genetic Resources and Crop Evolution, (DOI 10.1007/s10722-012-9903-x; Most Downloaded Article)http://www.springerlink.com/content/94l6858w1273g472/

Nageswara-Rao M,Kwit C, Stewart Jr. CN, 2012. Grass to solve global fuel crisis, 30th August 2012, BioSpectrum Asia, http://www.biospectrumasia.com/biospectrum/opinion/3439/grass-solve-global-petrol-scarcity

Salgotra, R.K. B.B. Gupta, R.J. Millwood, M. Balasubramaniam, and C.N Stewart, Jr. 2012. Introgression of bacterial leaf blight resistance and aroma genes using functional marker-assisted selection in rice (Oryza sativa L.). Euphytica, 187: 313-323.

Fu, C., R. Sunkar, C. Zhou, H. Shen, J. Zhang, J. Wolf, Y. Tang, D. G.J. Mann, C. N. Stewart, Jr., M. Udvardi, R. A. Dixon, and Z.-Y. Wang. 2012. Overexpression of miR156 in switchgrass (Panicum virgatum L.) results in various morphological alterations and leads to improved biomass production. Plant Biotechnology Journal, 10:443-452.

Zhao, N., J.-L. Ferrer, H.S. Moon, J. Kapteyn, X. Zhuang, M. Hasebe, C.N. Stewart Jr., D.R. Gang, and F. Chen. 2012. A SABATH methyltransferase from the moss Physcomitrella patens catalyzes S-methylation of thiols and has a role in detoxification. Phytochemistry, 81: 31-41.

Joyce, B. and C.N. Stewart, Jr. 2012. Designing the perfect plant feedstock for biofuel production: using the whole buffalo to diversify fuel and products. Biotechnology Advances, 30: 1011-1022.

Liu, Y., Z. Tang, H. Darmency, C. N. Stewart, Jr.,, K. Di, W. Wei, K. Ma.  2012. The effects of seed size on hybrids formed between oilseed rape (Brassica napus) and wild brown mustard (Brassica juncea). PLoS ONE, 7(6) e39705. http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0039705


Mann, D.G.J., L.L. Abercrombie, M.R. Rudis, R.J. Millwood, J.R. Dunlap, C.N. Stewart, Jr. 2012. Very bright orange fluorescent plants: endoplasmic reticulum targeting of orange fluorescent proteins as visual reporters in transgenic plants. BMC Biotechnology, 12:17 doi:10.1186/1472-6750-12-17 http://www.biomedcentral.com/1472-6750/12/17/abstract

Burris, K.P., P.M Davidson, C.N. Stewart, Jr., S. Zivanovic, F.M. Harte. 2012. Aqueous extracts of Yerbe Mate (Ilex paraguariensis) as a natural antimicrobial agent against Escherichia coli O157:H7 in a microbiological medium and pH 6 apple juice. Journal of Food Protection, 75: 753-757. 

Sun, G., C.N. Stewart, Jr., P. Xiao, B. Zhang. 2012. MicroRNA expression analysis in the cellulosic biofuel crop switchgrass (Panicum virgatum) under abiotic stress. PLoS ONE, 7(3): e32017.  doi:10.1371/journal.pone.0032017  http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0032017

Kwit, C. and C.N. Stewart, Jr. 2012. Gene flow matters in switchgrass (Panicum virgatum L.), a potential widespread biofuel feedstock. Ecological Applications, 22: 3-7.

Mann, D.G.J., P.R. LaFayette, L. L. Abercrombie, Z.R. King, M. Mazarei, M.C. Halter, C.R. Poovaiah, H. Baxter, H. Shen, R.A. Dixon, W.A. Parrott, C.N. Stewart, Jr. 2012. Gateway-compatible vectors for high-throughput gene functional analysis in switchgrass (Panicum virgatum L.) and other monocot species.  Plant Biotechnology Journal, 10:226-236.

Shen, H., X. He, C.R. Poovaiah, W.A. Wuddineh, J. Ma, D.G.J. Mann, H. Wang, L. Jackson, Y. Tang, C.N. Stewart, Jr., F. Chen, and R.A. Dixon. 2012 Functional characterization of the switchgrass (Panicum virgatum) R2R3-MYB transcription factor PvMYB4 for improvement of lignocellulosic feedstocks. New Phytologist, 193: 121-136.

2011 Publications


Liu, W., M Mazarei, M.R. Rudis, M.H. Fethe, and C.N. Stewart, Jr.2011. Rapid in vivo analysis of synthetic promoters for pathogen phytosensing. BMC Biotechnology, 11:108  http://www.biomedcentral.com/1472-6750/11/108

Mazarei, M. W. Liu, H. Al-Ahmad, P.R. Arelli, V.R. Pantalone, C.N. Stewart, Jr. 2011. Gene expression profiling of resistant and susceptible soybean lines infected with soybean cyst nematode. Theoretical and Applied Genetics, 12: 1193-1206.

Mazarei, M., H. Al-Ahmad, M.R. Rudis, B. L. Joyce, C.N. Stewart, Jr. 2011.  
Switchgrass (Panicum virgatum L.) cell suspension cultures: establishment, characterization, and application. Plant Science, 6: 712-715.

Mann, D.J.G., Z. King, W. Liu, B.L. Joyce, R.J. Percifield, J.S. Hawkins, P.R. LaFayette,  B.J. Artelt, J.N. Burris, M. Mazarei, J.L. Bennetzen, W.A. Parrott, C.N. Stewart, Jr. 2011. Switchgrass (Panicum virgatum L.) polyubiquitin gene (PvUbi1 and PvUbi2) promoters for use in plant transformation. BMC Biotechnology, 11:74 http://www.biomedcentral.com/1472-6750/11/74/

Vasudev, M., T.-C. Wu, M. Datta, M.A. Stroscio, S. Guthrie, M. Reed, K.P. Burris, C. N. Stewart, Jr. 2011. Optoelectric signatures of DNA-based hybrid nanostructures. IEEE Transactions on Nanotechnology, 10: 35-43.

Xia L., S.C. Lenaghan, M.  Zhang, Y.Wu, X. Zhao, J. N. Burris, C. N. Stewart, Jr. 2011. Characterization of English ivy (Hedera helix) adhesion force and imaging using atomic force microscopy. Journal of Nanoparticle Research, 13:1029-1037.

Salgotra, R.K., R. J. Millwood, S. Agarwal, C. N. Stewart, Jr, 2011. High-throughput functional marker assay for detection of Xa/xa and fgr genes in rice (Oryza sativa L.). Electrophoresis, 32: 2216-2222. 
Kwit, C. H.S. Moon, S.I. Warwick, C.N. Stewart, Jr. 2011. Transgene introgression in crop relatives: molecular evidence and mitigation strategies. Trends in Biotechnology, 29: 284-293.

Moon, H.S., L.L. Abercrombie, S. Eda, R. Blanvillain, J.G. Thomson, D.W. Ow, C.N. Stewart, Jr. 2011. Transgene excision in pollen using a codon optimized serine resolvase CinH-RS2 site-specific recombination system. Plant Molecular Biology, 75:621-631.

Jiang, H., W. Wei, X. Song, C.N. Stewart, Jr., G. Zhou, Z. Jiang. Q Zhu, S. Yu, S. Peng, 2011. Hyperspectral studies of  transgenic oilseed rape. International Journal of Remote Sensing, 32: 1095-1103.

Leckie, B.L., C.N. Stewart, Jr. 2011. Agroinfiltration as a technique for rapid assays for expressing candidate insect resistance transgenes in plants. Plant Cell Reports, 30:325-334.

Mann, D.G.J, P.R. LaFayette, L.G Abercrombie, W.A. Parrott, C.N. Stewart, Jr. 2011.
pANIC:  A versatile set of Gateway-compatible vectors for gene overexpression and RNAi-mediated down-regulation in monocots. Pp. 161-168 In: Stewart, C.N., Jr., A. Touraev, V. Citovsky, T. Tzfira (Eds). 2011. Plant Transformation Technologies. Wiley-Blackwell Scientific Publishing, Ames Iowa.

Moon, H.S., S. Eda, A.M. Saxton, D. Ow, C.N. Stewart, Jr. 2011. An efficient and rapid transgenic pollen screening and detection method using flow cytometry. Biotechnology Journal, 6:118-123.http://onlinelibrary.wiley.com/doi/10.1002/biot.201000258/pdf

2010 Publications


Himanen,S.J., A.-M. Nerg, G.M. Poppy C.N. Stewart, Jr., J.K. Holopainen. 2010. Abiotic stress and transgenics: implications for reproductive success and crop-wild gene flow in Brassicas. Basic and Applied Ecology, 11: 513-521.

Moon, H.S., J.M. Abercrombie, A.P. Kausch, C.N Stewart, Jr. 2010. Sustainable use of biotechnology for bioenergy feedstocks. Environmental Management, 46:531-538.

Riggins, C.W., Y. Peng, C. N. Stewart, Jr., and P. J. Tranel. 2010. Characterization of de novo waterhemp (Amaranthus tuberculatus) transcriptome using GS-FLX 454 pyrosequencing and its application for studies of herbicide target-site genes. Pest Management Science, 66: 1042-1052.

Peng, Y., L.L.G. Abercrombie, J.S. Yuan, C.W. Riggins, R.D. Sammons, P.J. Tranel, and C.N. Stewart, Jr. 2010.  Characterization of the horseweed Conyza canadensis transcriptome using GS-FLX 454 pyrosequencing and its application for expression analysis of candidate non-target herbicide-resistance genes. Pest Management Science, 66: 1053-1062.

Rao, M.R., H.S. Moon, T.M.H. Schenk, D. Becker, M. Mazarei and C.N. Stewart Jr. 2010. FLP/FRT recombination from yeast: application of a two gene cassette scheme as an inducible system in plants. Sensors, 10: 8526-8535; doi:10.3390/s100908526. 

Kang, B-g., X. Ye, L.D. Osburn, C.N. Stewart, Jr., Z-M. Cheng. 2010. Transgenic hybrid aspen overexpressing the Atwbc19 gene encoding an ATP binding cassette transporter confers resistance to four aminoglycoside antibiotics. Plant Cell Reports, 29:643-650.

Yuan, J.S., L.G. Abercrombie, Y. Cao, M.D. Halfhill, X. Zhou, Y. Peng, J. Hu, M.R. Rao, G.R. Heck, T.J. Larosa, R. D. Sammons, R. N. Trigiano, X. Wang, P. Ranjan, D. H. Johnson, P. A. Wadl, B.E. Scheffler, T.A Rinehart, R.N. Trigiano, C. N. Stewart, Jr. 2010. Functional genomics analysis of horseweed (Conyza canadensis) with special reference to the evolution of non-target-site glyphosate resistance. Weed Science, 58: 109-117.

Kausch, A.P., J. Hague, M. Oliver, Y. Li. H. Daniell, P. Maschia, L.S. Watrud, and C.N. Stewart, Jr. 2010. Transgenic biofuel feedstocks and strategies for bioconfinement. Biofuels,
1:163-176.

Millwood, Reginald J., H. S. Moon, and C. N. Stewart Jr. 2010. Fluorescent proteins in Transgenic plants. Reviews in Fluorescents, 387 – 403. 

Moon, H.S., Y. Li., C.N. Stewart, Jr. 2010. Keeping the genie in the bottle: transgene biocontainment by excision in pollen. Trends in Biotechnology, 28: 3-8.bullet(with accompanying journal cover)

Stewart, C.N., Jr., L. Shugart, G.-S. Liu, J. Zhuang, Y. Ma, G.A. Tuskan, R. Meilan, R.W. Gentry, G.S. Sayler. 2010. China-U.S. workshop on biotechnology of bioenergy plants.  Ecotoxicology, 19: 1-3.

2009 Publications: 


Mann, D.J.G., N. Labbé, R. Sykes, K. Reichel, L. Kline, I.M. Swamidoss, J.N. Burris, M. Davis, C.N. Stewart, Jr. 2009. Rapid assessment of lignin content and structure in switchgrass (Panicum virgatum L.) grown under different environmental conditions. BioEnergy Research, 2: 246-256.

Burris, J.N., D.J.G. Mann, B.L. Joyce, C.N. Stewart, Jr. 2009. An improved tissue culture system for embyrogenic callus production and plant regeneration in switchgrass (Panicum virgatum L.). BioEnergy Research, 2: 267-274.

Rose, C.W., R.J. Millwood, H.S. Moon, M.R. Rao, M.D. Halfhill, P.L. Raymer, S.I. Warwick, H. Al-Ahmad, J. Gressel, and C.N. Stewart, Jr. 2009. Genetic load and transgenic mitigating genes in transgenic Brassica rapa (field mustard) × Brassica napus (oilseed rape) hybrid populations. BMC Biotechnology, 9: 93  http://www.biomedcentral.com/1472-6750/9/93/

Chen, F., H. Al-Ahmad, B. Joyce, N. Zhao, T. G. Köllner, J. Degenhardt, C. N. Stewart, Jr. 2009. Within-plant distribution and emission of sesquiterpenes from Copaifera officinalis. Plant Physiology and Biochemistry, 47: 1017-1023.

Zhou, X., Z. Su, R.D. Sammons, Y. Peng, P.J. Tranel, C.N. Stewart, Jr., J.S. Yuan. 2009. Novel software package for cross-platform transcriptome analysis (CPTRA). BMC Bioinformatics, 10(Suppl 11)S16 http://www.biomedcentral.com/qc/1471-2105/10/S11/S16/

Xu, Z, D. Zhang, J. Hu, X. Zhou, X. Ye, K.L. Reichel, N.R. Stewart, R.D. Syrenne, X. Yang, P. Gao, W. Shi, C. Doeppke, R. W. Sykes, J.N. Burris, J. Bozell, Max Z.-M.. Cheng, D.G. Hayes, N. Labbe, M. Davis, C.N. Stewart, Jr., J.S. Yuan. 2009. Comparative genome analysis of lignin biosynthesis gene families across the plant kingdom. BMC Bioinformatics,  10(Suppl 11)S3 http://www.biomedcentral.com/1471-2105/10/S11/S3

Stewart, C.N., Jr., P.J. Tranel, D.P. Horvath, J.V. Anderson, L.H. Rieseberg, J.H. Westwood, C.A. Mallory-Smith, M.L. Zapiola, K.M. Dlugosch. 2009. Evolution of weediness and invasiveness: charting the course for weed genomics. Weed Science, 57: 451-462.

Rao, M.R., M.D. Halfhill, L.G Abercrombie, P. Ranjan, J.M. Abercrombie, J.S. Gouffon, A.M. Saxton, and C.N. Stewart, Jr. 2009. Phytoremediation and phytosensing of chemical
contaminants, RDX and TNT: identification of the required target genes
. Functional and Integrative Genomics, 9:537-547.

Di, K., C.N. Stewart, Jr.,W. Wei, B. Shen, Z.-X. Tang, K.-P. Ma. 2009. Fitness and maternal effects in hybrids formed between transgenic oilseed rape (Brassica napus L.) and wild brown mustard [B. juncea (L.) Czern et. Coss.] in the field. Pest Management Science, 65:753-760.

Yuan, J.S., S. Himanen, J.K. Holopainen, F. Chen, and C.N. Stewart, Jr. 2009. Smelling climate change: plant volatile organic compounds in changing environments.  Trends in Ecology and Evolution 24: 323-331.

Himanen, S., A.-M. Nerg A. Nissinen, D. M. Pinto, C.N. Stewart, Jr., G.M. Poppy, and J.K. Holopainen, 2009. Effects of elevated carbon dioxide and ozone on volatile terpenoid emissions and multitrophic communication of transgenic insecticidal oilseed rape (Brassica napus).  New Phytologist 181: 174-186.

Panthee, D.R.., J.J. Marois, D.L. Wright,  D. Narváez, J.S. Yuan, and C.N. Stewart Jr. 2009. Differential expression of genes in soybean in response to the causal agent of Asian soybean rust, (Phakopsora pachyrhizi Sydow), is soybean growth stage-specific. Theoretical and Applied Genetics, 118: 359-370

Himanen S.J., A.M. Nerg, A. Nissinen, C.N. Stewart, Jr., G.M. Poppy and J.K. Holopainen. 2009. Elevated atmospheric ozone increases concentration of insecticidal Bacillus thuringiensis (Bt) Cry1Ac protein concentrations in Bt Brassica napus and reduces feeding of a Bt target herbivore on the non-transgenic parent. Environmental Pollution, 157:181-185. 

2008 Publications


Himanen, S., A. Nissinen, W.-X. Dong, A.-M. Nerg, C.N. Stewart, Jr., G.M. Poppy, and J.K. Holopainen. 2008. Interactions of elevated carbon dioxide and temperature with aphid feeding on transgenic oilseed rape: Are Bacillus thuringiensis (Bt) plants more susceptible to nontarget herbivores in future climate? Global Change Biology, 14: 1437-1454.
  
Stewart, C.N., Jr. 2008. Pharming in crop communities. Nature Biotechnology, 26:1222-1223.

Abercrombie, J.M., M.D. Halfhill, P. Ranjan, M.R. Rao, A. Saxton, J.S. Yuan, and C.N. Stewart, Jr. 2008. Transcriptional responses of Arabidopsis thaliana to As(V) stress. BMC Plant Biology, 8:87 (http://www.biomedcentral.com/1471-2229/8/87 ).

Yuan, J.S., K.H. Tiller, H. Al-Ahmad, N.R. Stewart, C.N. Stewart, Jr. 2008. Plants to power: bioenergy to fuel the future. Trends in Plant Science, 13: 421-429. 

Stewart, C.N., Jr. (Ed.) 2008. Plant Biotechnology and Genetics: Principles, Techniques and Applications, Wiley and Sons, New York, 400 pp. June 2008.

Yuan, J.S., D. W. Galbraith, S. Y. Dai, P. Griffin and C.N. Stewart, Jr. 2008. Plant systems biology comes of age. Trends in Plant Science, 13:165-171

Mazarei, M.H., I. Teplova, M. R. Hajimorad and C.N. Stewart, Jr. 2008. Pathogen phytosensing: plants to report plant pathogens. Sensors, 8:2628-2641.

Mazarei, M.H., H. Al-Ahmad, M. R. Rudis and C.N. Stewart, Jr. 2008. Protoplast isolation and transient gene expression in switchgrass, Panicum virgatum L. Biotechnology Journal, 3:354-359.

Burris, K.A., A. Mentewab, S. Ripp, and C.N. Stewart, Jr. 2008. An Arabidopsis thaliana ABC transporter that confers kanamycin resistance in transgenic plants does not endow resistance to Escherichia coli. Microbial Biotechnology, 1:191-195.

Stewart, C.N., Jr. and L. E. Edwards. 2008. How to teach research ethics. The Scientist, 22:27.

Yuan, J., D. Wang. and C. N. Stewart, Jr. 2008. Statistical methods for efficiency adjusted real-time PCR analysis. Biotechnology Journal, 3:112-123.

2007 Publications


Yancey, A. and C. N. Stewart, Jr. 2007. Are university researchers at risk for patent infringement? Nature Biotechnology, 25:1225-1228.

Yuan, J.S., J. Burris, N. R. Stewart, A. Mentewab and C. N. Stewart, Jr. 2007. Statistical tools for transgene copy number estimation based on real-time PCR. BMC Bioinformatics, 8(Suppl 2): S6 doi:10.1186/1471-2105-8-S7-S6.

Panthee, D.R., J. Yuan, D.L. Wright, J.J. Marois, D. Mailhot and C. N. Stewart, Jr. 2007. Gene expression analysis in soybean in response tothe casual agent of Asian soybean rust Phakospora pachyrhizi Sydow) in an early growth stage. Functional and Integrative Genomics, 7:291-301. Appels Figure

Moon, H.S., M.D. Halfhill, L. L. Good, P. L. Raymer and C. N. Stewart, Jr. 2007. Characterization of directly transformed weedy Brassica rapa and introgressed B. rapa with Bt crylAc and gfp genes. Plant Cell Reports, 26:1001-1010.

Luo, K., H. Duan, D. Zhao, X. Zheng, W. Deng, Y. Chen, C. N. Stewart, Jr., R. McAvoy, X. Jiang, Y. Wu, A. He, Y. Pei and Y. Li. 2007. ‘GM-gene-deletor’: fused loxP-FRT recognition sequences dramatically improve the efficiency of FLP or CRE recombinase on transgene excision from pollen and seed of tobacco plants. Plant Biotechnology Journal, 5:263-274.

Stewart, C.N., Jr. 2007. Biofuels and biocontainment. Nature Biotechnology. 25:283-284.

Halfhill, M.D., L.L. Good, C. Basu, J. Burris, C.L.Main, T.C. Mueller, and C.N. Stewart, Jr. 2007. Transformation and segregation of GFP fluorescence and glyphosate resistance in horseweed (Conyza canadensis) hybrids. Plant Cell Reports, 26:303-311.

Yuan, J.S., P.J. Tranel, and C.N. Stewart, Jr. 2007. Non-target site herbicide resistance: a family business. Trends in Plant Science, 12:6-13.

Darbani, B., A. Eimanifar, C.N. Stewart, Jr., W. Camargo. 2007. Methods to produce marker-free transgenic plants. Biotechnology Journal, 2:83-90.

2006 Publications


Moon, H.S, M.D. Halfhill, L.C. Hudson, R.J. Millwood, and C.N. Stewart, Jr. 2006. Expression of green fluorescent protein in pollen of oilseed rape (Brassica napus L.) and its utility for assessing pollen movement in the field. Biotechnology Journal, 1:1147-1152.

Ferry, N., E.A. Mulligan, C.N. Stewart, B.E. Tabashnik, G.R. Port, and A.M.R. Gatehouse. 2006. Prey-mediated effects of transgenic canola on a beneficial, non-target, carabid beetle. Transgenic Research, 15:501-514.

Yuan, J.S., A. Reed, F. Chen, C.N. Stewart, Jr. 2006. Statistical analysis of real-time PCR data. BMC Bioinformatics, 7:85.

Stewart, C.N., Jr. 2006. Go with the glow: fluorescent proteins to light transgenic organisms. Trends in Biotechnology, 24:155-162. 

Markham, K., T. Chalk, and C.N. Stewart, Jr. 2006. Evaluation of protein-based defenses against phytophagous insects in ferns and mosses. International Journal of Plant Science, 167:111-117.

2005 Publications


Stewart, C.N., Jr., R.J. Millwood, M.D. Halfhill, M. Ayalew, V. Cardoza, M. Kooshki, G.A.Capelle, K.R. Kyle, D. Piaseki, G. McCrum, J. Di Benedetto. 2005. Laser-induced fluorescence imaging and spectroscopy of GFP transgenic plants. Journal of Fluorescence, 15:697-705. 

Wei, W., T.H. Schuler, S.J. Clark, C.N. Stewart, Jr., and G. M. Poppy. 2005. Age-related increase in levels of insecticidal protein in the progenies of transgenic oilseed rape and its efficacy against a susceptible strain of diamondback moth. Annals of Applied Biology, 147:227-235. 

Mentewab, A. and C. N. Stewart, Jr. 2005. Overexpression of an Arabidopsis thaliana ABC transporter confers kanamycin resistance to transgenic plants. Nature Biotechnology, 23:1177-1180. Supplement

Halfhill, M.D.; Sutherland, J.P.; Moon, H.S.; Poppy, G.M.; Warwick, S.I.; Weissinger, A.K.; Rufty, T.W.; Raymer, P.L. and C. Neal Stewart, Jr. 2005. Growth, productivity, and competitiveness of introgressed weedy Brassica rapa hybrids selected for the presence of Bt cry1Ac and gfp transgenes. Molecular Ecology, 14:3177-3189.

C. Neal Stewart, Jr. 2005. Monitoring the presence and expression of transgenes in living plants. TRENDS in Plant Science, 10(8):390-396.

Schuler, T. H.; Clark, A.J.; Clark, S. J.; Poppy, G.M.; C. N. Stewart, Jr. and I. Denholm 2005. Laboratory studies of the effects of reduced prey choice caused by Bt plants on a predatory insect. Bulletin of Entomological Research, 95:243-247.

Warwick, S.I. and C. N. Stewart, Jr. 2005. Crops Come from Wild Plants – Domestication, Transgenes, and Linkage Together Shape Ferality. pp 9-30 In: J. Gressel (Ed.) Crop Ferality and Volunteerism. CRC Press, Boca Raton, Florida.

Mentewab A..; V. Cardoza and C. N. Stewart, Jr. 2005. Genomic analysis of the response of Arabidopsis thaliana to trinitrotoluene as revealed by cDNA microarrays. Plant Science, 168:1409-1424.

2004 Publications


Cardoza, V. and C. N. Stewart, Jr. 2004. Brassica Biotechnology: progress in cellular and molecular biology. In Vitro Cell. Dev. Biol. – Plant, 40:542-551.

Halfhill, M.D., R. J. Millwood, and C. N. Stewart, Jr. 2004. GFP Quantification in whole plants. pp. 215-226. In: L. Pena (Ed). Transgenic Plants: Methods and Protocols. Methods in Molecular Biology. Humana Press, Totawa, NJ.

Hudson, L.C., M.D. Halfhill, and C. N. Stewart, Jr. 2004. Transgene dispersal through pollen. pp. 365-374 In: L. Pena (Ed). Transgenic Plants: Methods and Protocols. Methods in Molecular Biology. Humana Press, Totawa, NJ.

Basu C, Halfhill MD, Mueller TC, Stewart CN. 2004. Weed genomics: new tools to understand weed biology. Trends in Plant Science, 9: 391-398.

Halfhill MD, Zhu B, Warwick SI, Raymer PL, Millwood RJ, Weissinger AK, Stewart CN. 2004. Hybridization and backcrossing between transgenic oilseed rape and two related weed species under field conditions. Environ. Biosafety Res., 3: 73-81.

Cardoza V and Stewart, C.N., Jr. 2004. Agrobacterium-mediated transformation of canola. pp 379-387 In: I. S. Curtis (Ed.) Transgenic Crops of the World – Essential Protocols Kluwer Academic Publishers, Dordrecht.

Patel N, Cardoza V, Christensen E, Rekapalli B, Ayalew M, Stewart, CN. 2004. Differential gene expression of Chlamydomonas reinhardtii in response to 2,4,6-trinitrotoluene (TNT) using microarray analysis. Plant Sciences, 167: 1109-1122.

Stewart, C.N., Jr. 2004. Genetically Modified Planet: Environmental Impacts of Genetically Engineered Plants. Oxford University Press, New York. 240 pp. 

Zhu, B., Lawrence, J.R., Warwick, S.I., Mason, P., Braun, L., Halfhill, M.D., and Stewart, C.N. Jr. 2004. Inheriteance of GFP-Bt transgenes from Brassica napus in backcrosses with three wild B. rapa accessions. Environ. Biosafety Res., 3:45-54.

Hudson, L.C., and Stewart, C.N. Jr. 2004. Effects of pollen-synthesized green fluorescent protein on pollen grain fitness. Sex Plant Reprod., 17:49-53.

Zhu, J., Lawrence, S.I., Warwick, P. Mason, Braun, L., Halfhill, M.D., and Stewart, C. N. Jr. 2004. Stable Bacillus thuringiensis (Bt) toxin content in interspecific F1 and backcross populations of wild Brassica rapa after Bt gene transfer. Molecular Ecology, 13:237-241.

2003 Publications


Mason, P., Braun, L., Warwick, S. I., Zhu, B., and Stewart, Jr. C.N. 2003. Transgenic Bt-producing Brassica napusPlutella xylostella selection pressure and fitness of weedy relatives. Environ. Biosafety Res., 2:263-276.

Halfhill, M.D., Millwood, R.J., Rufty, T.W., Weissinger, A.K. and Stewart, C.N. Jr. 2003. Spatial and temporal patterns of green fluorescent protein (GFP) fluorescence during leaf canopy development in transgenic oilseed rape, Brassica napus L. Plant Cell Rep., 22:338-343.

Halfhill, M.D., Millwood, R.J., Weissinger, Warwick, S.I., Stewart, C. N. 2003. Additive transgene expression and genetic introgression in multiple green-fluorescent protein transgenic crop x weed hybrid generations. Theor Appl. Genet., 107:1533-1540.

Stewart, C. Neal, Jr., Halfhill, M.D., Warwick, S.I. 2003. Transgene introgression from genetically modified crops to their wild relatives. Nature Reviews Genetics, 4:806-817. 

Warwick, S.I., Simard, M.J., Legre, A., Beckie, H.J., Braun, L., Zhu, B., Mason, P., Seguin-Swartz, G., Stewart, C. N. Jr. 2003. Hybridization between transgenic Brassica napus L. and its wild relatives: Brassica rapa L., Raphanus raphanistrum L., Sinapis arvensis L., and Erucastrum gallicum (Willd.) O. E. Schulz. Theor Appl Genet, 107:528-539. 

Richards, H.A., Halfhill, M.D., Millwood, R.J., Stewart, C. N. Jr. 2003. Quantitative GFP fluorescence as an indicator of recombinant protein synthesis in transgenic plants. Plant Cell Rep., 22:117-121. 

Kooshki, M., Mentewab, A., Stewart, C. Neal, Jr. 2003. Pathogen inducible reporting in transgenic tobacco using a GFP construct. Plant Science, 165:213-219. 

Mueller, T.C., Massey, J.H., Hayes, R.M., Main, C.L., and Stewart, C.N. Jr. 2003. Shikimate accumulates in both glyphosate-sensitive and glyphosate-resistant horseweed (Conyza canadensis L. Cronq). J. Agric. Food. Chem., 51:680-684. 

Schuler, T.H., Potting, R.P.J., Denholm, I., Clark, S.J., Clark, A.J., Stewart, C.N. Jr., and Poppy, G.M. 2003. Tritrophic choice experiments with Bt plants, the diamondback moth (Plutella xylostella) and the parasitoid Cotesia plutellae. Transgenic Research, 12:351-361. 

Stewart, C. N. Jr. 2003. Press before paper-when media and science collide. Nature Biotechnology, 21:353-354.

Stewart, C.N., Jr. 2003. Transgenic Plants: Current Innovations and Future Trends (Editor). Horizon Scientific Press. Wymondham, UK. 297 pp. 

Millwood, R.J., M.D. Halfhill, D. Harkins, R. Rusotti, and C.N. Stewart, Jr. 2003. Instrumentation and methodology for quantifying GFP fluorescence in intact plant organs . BioTechniques, 34:638-643.

Cardoza, V. and C.N. Stewart, Jr. 2003. Increased Agrobacterium-mediated transformation and rooting efficiencies in canola Brassica napus L. from hypocotyl segment explants. Plant Cell Reports, 21:599-604. 

Stewart, C.N., Jr. 2003. (Ed.) Transgenic Plants: Current Innovations and Future Trends. Horizon Scientific Press. Wymondham, UK. 297 pp.

Richards, H. A., Han, C. T., Hopkins, R. G., Failla, M. L., Ward, W. W. and Stewart, C.N. Jr. 2003. Safety assessment of recombinant green fluorescent protein orally administered to weaned rats. J. Nutr., 133:1909-1912.

2002 Publications 


Tomlin, E.S., S.R. Branch, D. Chamberlain, H. Gabe, M.S. Wright, and C.N. Stewart, Jr. 2002. Screening of soybean, Glycine max (L.) Merrill, lines for somatic embryo induction and maturation capability from immature cotyledons. In Vitro Cell. Dev. Biol. – Plant., 38:543-548.

Halfhill, M.D., R.J. Millwood, P.L.Raymer and C.N. Stewart, Jr. 2002. Bt-transgenic oilseed rape hybridization with its weedy relative, Brassica rapa. Environ. Biosafety Res., 1:19-28. 

Stewart, C.N., Jr., M. D. Halfhill, and R. J. Millwood. 2002. Geen fluorescent protein in transgenic plants: Brassica transformation. Methods in Molecular Biology, 183(19):245-252. 

Stewart, C.N., Jr., and S. K. Wheaton. 2002. Urban myths and real risks of genetically enhanced (GE) crops. pp. 528-551. In: Plants, Genes, and Crop Biotechnology. Maarten J. Chrispeels and David E. Sadova, (Eds) Bartlett and Jones Publishers, Sudbury, Mass. 

2001 Publications


Pulliam, D.A., D.L. Williams, R.M. Broadway, and C.N. Stewart, Jr. 2001. Isolation and characterization of a serine proteinase inhibitor cDNA from cabbage and its antibiosis in transgenic tobacco plants. Plant Cell Biotechnology and Molecular Biology, 2 (1&2):19-32. 

Halfhill, M.D., H.A. Richards, S.A. Mabon, C.N. Stewart, Jr. 2001. Expression of GFP and Bt transgenes in Brassica napus and hybridization and introgression with Brassica rapa. Theoretical and Applied Genetics, 103:659-667. 

Hudson, L.C., D. Chamberlain, and C.N. Stewart, Jr. 2001. GFP-tagged pollen to monitor gene flow of transgenic plants. Molecular Ecology Notes, 1:321-324. 

Stewart, C. Neal Jr., Matthew D. Halfhill, Harold A. Richards. 2001. GFP in plant biotechnology and agriculture, pp. 453-456 in Bioluminescence and Chemiluminescence 2000, J F Case, PJ Herring, BH Robison, SHD Haddock, LJ Kricka, PE Stanley (Eds). World Scientific Publishing Company, Singapore. 

Stewart, C.N., Jr. 2001. The utility of green fluorescent protein in transgenic plants. Plant Cell Reports, 20:376-382. 

Stewart, C.N., Jr. 2001. Update to applications of green fluorescent protein in plants, pp. 144-145 in Q. Lu and M.P. Weiner (Eds.), Cloning and Expression Vectors for Protein Studies. Eaton Publishing, Westborough, Mass. 

Stewart, C.N., Jr., and S.K. Wheaton. 2001. GM crop data-agronomy and ecology in tandem. Nature Biotechnology, 19:3. 

2000 Publications 


Stewart, C.N., Jr., H.A. Richards, M.D. Halfhill. 2000 Transgenic plants and biosafety: science, onceptions and public perceptions. BioTechniques, 29:832-843. 

Harper, B.K., and C.N. Stewart, Jr. 2000. Patterns of green fluorescent protein in transgenic plants. Plant Molecular Biology Reporter, 18: 141a-141i. 

Ramachandran, S., G.D. Buntin, J.N. All, P.L. Raymer and C.N. Stewart, Jr. 2000. Intraspecific competition of an insect-resistant transgenic canola in seed mixtures. Agronomy Journal, 92:368-374. 

Publications from 1999 and Earlier


Harper, B.K., S.A. Mabon, S.M. Leffel, M.D. Halfhill, H.A. Richards, K.A. Moyer, and C.N. Stewart, Jr. 1999. Green fluorescent protein in transgenic plants indicates the presence and expression of a second gene. Nature Biotechnology, 17:1125-1129.

Chamberlain, D and C.N. Stewart, Jr. 1999. Transgene escape and transplastomics. Nature Biotechnology, 17:330-331 Invited Commentary.

Stewart, C.N., Jr. 1999. Insecticidal transgenes into nature: gene flow, ecological effects, relevancy and monitoring. Pp: 179-190 in, Symposium Proceedings No. 72 Gene Flow and Agriculture-Relevance for Transgenic Crops (ed P.J.W. Lutman) Proceedings of a Symposium held at the University of Keele, 12-14 April 1999. British Crop Protection Council, Surrey, UK.

Ramachandran, S., G.D. Buntin, J.N. All, B.E. Tabashnik, P.L. Raymer M.J. Adang, D.A. Pulliam, and C.N. Stewart, Jr. 1998. Survival, fitness, and oviposition of resistant diamondback moth (Lepidoptera: Plutellidae) on transgenic canola producing a Bacillus thuringiensis toxin. Journal of Economic Entomology, 91:1239-1244.

Ramachandran, S., G.D. Buntin, J.N. All, P.L. Raymer, and C.N. Stewart, Jr. 1998. Movement and survival of diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae) larvae in mixtures of non-transgenic and transgenic canola containing a Bacillus thuringiensis cryIA(c) gene. Environmental Entomology, 27:649-656.

Ramachandran, S., G.D. Buntin, J.N. All, P.L. Raymer, and C.N. Stewart, Jr. 1998. Greenhouse and field evaluations of transgenic canola against diamondback moth, Plutella xylostella and corn earworm, Helicoverpa zea. Entomologia Experimentalis et Applicata 88:17-24.

Stewart, C.N., Jr. 1997. Rapid DNA extraction from plants. pp. 25-28 in: M.R. Micheli and R. Bova (eds.) Fingerprinting Methods Based on Arbitrarily Primed PCR, Springer Verlag, Heidelberg.

Leffel, S.M., S.A. Mabon, and C.N. Stewart, Jr.1997. Applications of green fluorescent protein in plants. BioTechniques, 23: 912-918. 

Stewart, C.N., Jr., J.N. All, P.L Raymer, S. Ramachandran. 1997. Increased fitness of transgenic insecticidal rapeseed under insect selection pressure. Molecular Ecology, 6:773-779.

Stewart, C.N., Jr. 1996. Transgene flow and persistence may be monitored using in vivo markers such as GFP. Biosafety Volume 2, (BY96003), September 2nd 1996. Refereed Online Journal, URL – http://www.bdt.org.br/bioline/by.

Stewart, C.N., Jr. 1996. Monitoring transgenic plants with in vivo markers. Nature Biotechnology, 14:682.

Stewart, C.N., Jr., M.J. Adang, J.N. All, H.R. Boerma, G. Cardineau, and W.A. Parrott. 1996. Genetic transformation, recovery, and characterization of transgenic soybean for synthetic Bacillus thuringiensis cryIA(c). Plant Physiology, 112:121-129.

Stewart, C.N., Jr., M.J. Adang, J.N. All, P.L Raymer, S. Ramachandran, and W.A. Parrott. 1996. Insect control and dosage effects in transgenic canola, Brassica napus L. (Brassicaceae), containing a synthetic Bacillus thuringiensis cryIA(c) gene. Plant Physiology, 112:115-120.

Stewart, C.N.,Jr., and L. Excoffier. 1996. Assessing population genetic structure and variability with RAPD data: application to the American cranberry. Journal of Evolutionary Biology, 9:153-171.

Stewart, C.N., Jr., G. Rosson, B.W. Shirley, and D.M. Porter. 1996. Population genetic variation of rare and endangered Iliamna (Malvaceae) in Virginia. Biological Journal of the Linnean Society, 58:357-369.

Stewart, C.N., Jr., and E.T. Nilsen. 1995. Phenotypic plasticity and genetic variation of Vaccinium macrocarpon(American cranberry) I. Reaction norms of clones from central and marginal populations in a common garden.International Journal of Plant Sciences, 156:687-697. 

Stewart, C.N., Jr., and E.T. Nilsen. 1995. Phenotypic plasticity and genetic variation of Vaccinium macrocarpon(American cranberry) II. Reaction norms and spatial clonal patterns in two marginal populations. International Journal of Plant Sciences, 156:698-708.

Stewart, C.N., Jr., and D.M. Porter. 1995. The usefulness of RAPD profiling in biological conservation: an application to estimating clonal variation in rare and endangered Iliamna in Virginia. Biological Conservation, 74:135-142. 

Parrott, W.A., J.N. All, M.J. Adang, M.A. Bailey, H.R. Boerma, and C.N. Stewart, Jr. 1994. Recovery and evaluation of soybean (Glycine max [L.] Merr.) plants transgenic for a Bacillus thuringiensis var. kurstaki insecticidal gene. In Vitro Cellular and Developmental Biology-Plant, 30:144-149. 

Stewart, C.N., Jr., and E.T. Nilsen. 1993. Association of edaphic factors and vegetation in several isolated Appalachian peat bogs. Bulletin of the Torrey Botanical Club, 120:128-135.

Stewart, C.N., Jr., and E.T. Nilsen. 1993. Responses of Drosera capensis and D. binata var. multifida (Droseraceae) to manipulations of insect availability and soil nutrient levels. New Zealand Journal of Botany, 31:385-390.

Stewart, C.N., Jr., and L.E. Via. 1993. A rapid CTAB DNA isolation technique useful for RAPD fingerprinting and other PCR applications. BioTechniques, 14:748-751.

Stewart, C.N., Jr., and E.T. Nilsen. 1992. Drosera rotundifolia growth and nutrition in a natural population with special reference to the significance of insectivory. Canadian Journal of Botany, 70:1409-1416.